Wiley on Mass Spectrometry

Enables you to detect, identify, and characterize hundreds of drugs that may be used by athletes Mass spectrometry has become essential to sports drug testing. This book examines both the principles of sports drug testing and the use of mass spectrometry techniques and mass spectral data to detect, identify, and characterize hundreds of known and unknown drugs that athletes may use to enhance their performance. The author provides a detailed overview of the mass spectrometry of numerous classes of therapeutics and agents, various analyzers to detect low- and high-molecular weight drugs, as well as techniques to discriminate between endogenously produced and synthetically derived compounds. Mass Spectrometry in Sports Drug Testing begins with a full chapter dedicated to the history of sports drug testing. Next, the book provides the principles and techniques needed to maximize the specificity and sensitivity of mass spectrometric assays, including: • Detailed, step-by-step assays with sample preparation • Discussion of both chromatographic separation and mass spectrometric analysis • Characterization of analytes in order to unequivocally identify banned substances • Mass spectrometric behavior of low- and high-molecular weight analytes Throughout the book, descriptive examples illustrate the principles, advantages, and limitations of different assays. Mass Spectrometry in Sports Drug Testing not only sets forth the role mass spectrometry plays in detecting drug use among athletes, it also adds new insights into the health and ethical issues of doping in sports.

This book provides a detailed description of various multidimensional chromatographic separation techniques. The editor first provides an introduction to the area and then dives right into the various complex separation techniques. While still not used routinely comprehensive chromatography techniques will help acquaint the readers with the fundamentals and possible benefits of multi-dimensional separations coupled with mass spectrometry. The topics include a wide range of material that will appease all interested in either entering the field of multidimensional chromatography and those looking to gain a better understanding of the topic.

An insightful exploration of the key aspects concerning the chemical analysis of antibiotic residues in food. The presence of excess residues from frequent antibiotic use in animals is not only illegal but can pose serious health risks by contaminating products for human consumption such as meat and milk. Chemical Analysis of Antibiotic Residues in Food is a single-source reference for readers interested in the development of analytical methods for analyzing antibiotic residues in food. It covers themes that include quality assurance and quality control, antibiotic chemical properties, pharmacokinetics, metabolism, distribution, food safety regulations, and chemical analysis. In addition, the material presented includes background information valuable for understanding the choice of marker residue and target animal tissue to use for regulatory analysis. This comprehensive reference: • Includes topics on general issues related to screening and confirmatory methods • Presents updated information on food safety regulation based on routine screening and confirmatory methods, especially LC-MS • Provides general guidance for method development, validation, and estimation of measurement uncertainty “Chemical Analysis of Antibiotic Residues in Food” is written and organized with a balance between practical use and theory to provide laboratories with a solid and reliable reference on antibiotic residue analysis. Thorough coverage elicits the latest scientific findings to assist the ongoing efforts toward refining analytical methods for producing safe foods of animal origin.

Explores the impact of the latest breakthroughs in cluster SIMS technology. Cluster secondary ion mass spectrometry (SIMS) is a high spatial resolution imaging mass spectrometry technique, which can be used to characterize the three-dimensional chemical structure in complex organic and molecular systems. It works by using a cluster ion source to sputter desorb material from a solid sample surface. Prior to the advent of the cluster source, SIMS was severely limited in its ability to characterize soft samples as a result of damage from the atomic source. Molecular samples were essentially destroyed during analysis, limiting the method's sensitivity and precluding compositional depth profiling. The use of new and emerging cluster ion beam technologies has all but eliminated these limitations, enabling researchers to enter into new fields once considered unattainable by the SIMS method. With contributions from leading mass spectrometry researchers around the world, Cluster Secondary Ion Mass Spectrometry: Principles and Applications describes the latest breakthroughs in instrumentation, and addresses best practices in cluster SIMS analysis. It serves as a compendium of knowledge on organic and polymeric surface and in-depth characterization using cluster ion beams. It covers topics ranging from the fundamentals and theory of cluster SIMS, to the important chemistries behind the success of the technique, as well as the wide-ranging applications of the technology. Examples of subjects covered include: • Cluster SIMS theory and modeling • Cluster ion source types and performance expectations • Cluster ion beams for surface analysis experiments • Molecular depth profiling and 3-D analysis with cluster ion beams • Specialty applications ranging from biological samples analysis to semiconductors/metals analysis • Future challenges and prospects for cluster SIMS This book is intended to benefit any scientist, ranging from beginning to advanced in level, with plenty of figures to help better understand complex concepts and processes. In addition, each chapter ends with a detailed reference set to the primary literature, facilitating further research into individual topics where desired. Cluster Secondary Ion Mass Spectrometry: Principles and Applications is a must-have read for any researcher in the surface analysis and/or imaging mass spectrometry fields.

The definitive guide to mass spectrometry techniques in biology and biophysics The use of mass spectrometry (MS) to study the architecture and dynamics of proteins is increasingly common within the biophysical community, and Mass Spectrometry in Structural Biology and Biophysics: Architecture, Dynamics, and Interaction of Biomolecules, Second Edition provides readers with detailed, systematic coverage of the current state of the art. Offering an unrivalled overview of modern MS-based armamentarium that can be used to solve the most challenging problems in biophysics, structural biology, and biopharmaceuticals, the book is a practical guide to understanding the role of MS techniques in biophysical research. Designed to meet the needs of both academic and industrial researchers, it makes mass spectrometry accessible to professionals in a range of fields, including biopharmaceuticals. This new edition has been significantly expanded and updated to include the most recent experimental methodologies and techniques, MS applications in biophysics and structural biology, methods for studying higher order structure and dynamics of proteins, an examination of other biopolymers and synthetic polymers, such as nucleic acids and oligosaccharides, and much more. Featuring high-quality illustrations that illuminate the concepts described in the text, as well as extensive references that enable the reader to pursue further study, Mass Spectrometry in Structural Biology and Biophysics is an indispensable resource for researchers and graduate students working in biophysics, structural biology, protein chemistry, and related fields.

Facilitates the discovery and development of new, effective therapeutics. With coverage of the latest mass spectrometry technology, this book explains how mass spectrometry can be used to enhance almost all phases of drug discovery and drug development, including new and emerging applications. The book's fifteen chapters have been written by leading pharmaceutical and analytical scientists. Their contributions are based on a thorough review of the current literature as well as their own experience developing new mass spectrometry techniques to improve the ability to discover and develop new and effective therapeutics. “Mass Spectrometry for Drug Discovery and Drug Development” begins with an overview of the types of mass spectrometers that facilitate drug discovery and development. Next it covers: • HPLC—high-resolution mass spectrometry for quantitative assays • Mass spectrometry for siRNA • Quantitative analysis of peptides • Mass spectrometry analysis of biological drugs • Applications that support medicinal chemistry investigations • Mass spectrometry imaging and profiling Throughout the book, detailed examples underscore the growing role of mass spectrometry throughout the drug discovery and development process. In addition, images of mass spectra are provided to explain how results are interpreted. Extensive references at the end of each chapter guide readers to the primary literature in the field. “Mass Spectrometry for Drug Discovery and Drug Development” is recommended for readers in pharmaceutics, including medicinal chemists, analytical chemists, and drug metabolism scientists. All readers will discover how mass spectrometry can streamline and advance new drug discovery and development efforts.

The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets. The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease. This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases.

Reviews all the latest basic and clinical research findings. With contributions from leading international experts in the field, this book is dedicated to all facets of uremic toxins research, including low molecular weight solutes, protein-bound solutes, and middle molecules. Moreover, it covers everything from basic mass spectrometry research to the latest clinical findings and practices. Uremic Toxins is divided into three sections: • Section One, Uremic Toxins, explores the definition, classification, listing, and mass spectrometric analysis of uremic toxins • Section Two, Selected Uremic Toxins, describes key uremic toxins, explaining chemical structures, metabolism, analytical methods, plasma levels, toxicity, clinical implications, and removal methods. Among the uremic toxins covered are indoxyl sulfate, asymmetric dimethylarginine, PTH, ß2-microglobulin, and AGEs • Section Three, Therapeutic Removal of Uremic Toxins, describes how uremic toxins can be removed by hemodialysis, peritoneal dialysis, and oral sorbent All chapters are based on the authors' thorough review of the literature as well as their own personal laboratory and clinical experience. References at the end of each chapter provide a gateway to the literature in the field. Reviewing all the latest basic and clinical research findings, “Uremic Toxins” will help bench scientists in nephrology advance their own investigations. It will also help clinicians take advantage of the latest tested and proven treatments for the management of chronic kidney disease.

Introduces readers to the state of the art of omics platforms and all aspects of omics approaches for clinical applications This book presents different high throughput omics platforms used to analyze tissue, plasma, and urine. The reader is introduced to state of the art analytical approaches (sample preparation and instrumentation) related to proteomics, peptidomics, transcriptomics, and metabolomics. In addition, the book highlights innovative approaches using bioinformatics, urine miRNAs, and MALDI tissue imaging in the context of clinical applications. Particular emphasis is put on integration of data generated from these different platforms in order to uncover the molecular landscape of diseases. The relevance of each approach to the clinical setting is explained and future applications for patient monitoring or treatment are discussed. Integration of omics Approaches and Systems Biology for Clinical Applications presents an overview of state of the art omics techniques. These methods are employed in order to obtain the comprehensive molecular profile of biological specimens. In addition, computational tools are used for organizing and integrating these multi-source data towards developing molecular models that reflect the pathophysiology of diseases. Investigation of chronic kidney disease (CKD) and bladder cancer are used as test cases. These represent multi-factorial, highly heterogeneous diseases, and are among the most significant health issues in developed countries with a rapidly aging population. The book presents novel insights on CKD and bladder cancer obtained by omics data integration as an example of the application of systems biology in the clinical setting. • Describes a range of state of the art omics analytical platforms • Covers all aspects of the systems biology approach-from sample preparation to data integration and bioinformatics analysis • Contains specific examples of omics methods applied in the investigation of human diseases (Chronic Kidney Disease, Bladder Cancer) Integration of omics Approaches and Systems Biology for Clinical Applications will appeal to a wide spectrum of scientists including biologists, biotechnologists, biochemists, biophysicists, and bioinformaticians working on the different molecular platforms. It is also an excellent text for students interested in these fields.

Provides an overview of the use of mass spectrometry (MS) for the analysis of pesticide residues and their metabolites. • Presents state of the-art MS techniques for the identification of pesticides and their transformation products in food and environment • Covers important advances in MS techniques including MS instrumentation and chromatographic separations (e.g. UPLC, HILIC, comprehensive GCxGC) and applications • Illustrates the main sample preparation techniques (SPE, QuEChERS, microextraction) used in combination with MS for the analysis of pesticides • Describes various established and new ionization techniques as well as the main MS platforms, software tools and mass spectral libraries

Covers the area of lipidomics from fundamentals and theory to applications. • Presents a balanced discussion of the fundamentals, theory, experimental methods and applications of lipidomics • Covers different characterizations of lipids including Glycerophospholipids; Sphingolipids; Glycerolipids and Glycolipids; and Fatty Acids and Modified Fatty Acids • Includes a section on quantification of Lipids in Lipidomics such as sample preparation; factors affecting accurate quantification; and data processing and interpretation • Details applications of Lipidomics Tools including for Health and Disease; Plant Lipidomics; and Lipidomics on Cellular Membranes

Provides a single-source reference for readers interested in the development of analytical methods for analyzing non-antimicrobial veterinary drug residues in food. • Provides a comprehensive set of information in the area of consumer food safety and international trade • Covers general issues related to analytical quality control and quality assurance, measurement uncertainty, screening and confirmatory methods • Details many techniques including nanotechnology and aptamer based assays covering current and potential applications for non-antimicrobial veterinary drugs • Provides guidance for analysis of banned drugs including natural and synthetic steroids, Resorcylic acid lactones, and Beta-agonists

Provides a comprehensive description of mass spectrometry basics, applications, and perspectives Mass spectrometry is a modern analytical technique, allowing for fast and ultrasensitive detection and identification of chemical species. It can serve for analysis of narcotics, counterfeit medicines, components of explosives, but also in clinical chemistry, forensic research and anti-doping analysis, for identification of clinically relevant molecules as biomarkers of various diseases. This book describes everything readers need to know about mass spectrometry-from the instrumentation to the theory and applications. It looks at all aspects of mass spectrometry, including inorganic, organic, forensic, and biological MS (paying special attention to various methodologies and data interpretation). It also contains a list of key terms for easier and faster understanding of the material by newcomers to the subject and test questions to assist lecturers. Knowing how crucial it is for young researchers to fully understand both the power of mass spectrometry and the importance of other complementary methodologies, Mass Spectrometry: An Applied Approach teaches that it should be used in conjunction with other techniques such as NMR, pharmacological tests, structural identification, molecular biology, in order to reveal the true function(s) of the identified molecule. • Provides a description of mass spectrometry basics, applications and perspectives of the technique • Oriented to a broad audience with limited or basic knowledge in mass spectrometry instrumentation, theory, and its applications in order to enhance their competence in this field • Covers all aspects of mass spectrometry, including inorganic, organic, forensic, and biological MS with special attention to application of various methodologies and data interpretation • Includes a list of key terms, and test questions, for easier and faster understanding of the material Mass Spectrometry: An Applied Approach is highly recommended for advanced students, young scientists, and anyone involved in a field that utilizes the technique.

Monitoring Forest Damage with Mass Spectrometry-Based Metabolomics Methods Understand forest responses to climate change with this timely introduction Forests are among the most critical parts of our global ecosystem, responsible for the air we breathe, home to most of the earth's species, and crucial sources of food and raw materials. Forest development is therefore one of the most important areas of ecological study, and damage to forests is potentially existential. Metabolomics, a toolkit which accrues data on interactions between genetic and environmental conditions, promises to advance our understanding of how these vital ecosystems respond to dramatic changes in climate and environment. Monitoring Forest Damage with Mass Spectrometry-Based Metabolomics Methods offers a thorough, accessible discussion of metabolomic techniques and their applications in forest tree research. It promises to enrich the reader's understanding of how forests are being transformed by globe-spanning changes, and to arm researchers with tools for reacting to these potentially epochal developments. Monitoring Forest Damage with Mass Spectrometry-Based Metabolomics Methods readers will also find: • Analysis of specialized secondary metabolites such as phytohormones • Detailed discussion of ecologically important tree genera such as Pinus, Populus, Quercus, and many more • Supplementary materials related to study design, sample preparation, and instrumental analysis protocols Monitoring Forest Damage with Mass Spectrometry-Based Metabolomics Methods is ideal for researchers in analytical chemistry, mass spectrometry, metabolomics, forest research, the life sciences, and all other related fields.

• Covers all major modifications, including phosphorylation, glycosylation, acetylation, ubiquitination, sulfonation and and glycation • Discussion of the chemistry behind each modification, along with key methods and references • Contributions from some of the leading researchers in the field • A valuable reference source for all laboratories undertaking proteomics, mass spectrometry and post-translational modification research

Provides comprehensive coverage of the interpretation of LC–MS–MS mass spectra of 1300 drugs and pesticides • Provides a general discussion on the fragmentation of even-electron ions (protonated and deprotonated molecules) in both positive-ion and negative-ion modes • This is the reference book for the interpretation of MS–MS mass spectra of small organic molecules • Covers related therapeutic classes of compounds such as drugs for cardiovascular diseases, psychotropic compounds, drugs of abuse and designer drugs, antimicrobials, among many others • Covers general fragmentation rule as well as specific fragmentation pathways for many chemical functional groups • Gives an introduction to MS technology, mass spectral terminology, information contained in mass spectra, and to the identification strategies used for different types of unknowns

A guide for scientists, pediatricians and students involved in metabolic studies in pediatric research • Addresses the availability of modern analytical techniques and how to apply these techniques in metabolic studies • Covers the whole range of available mass spectrometric techniques used for metabolic studies including Stable Isotope Methodology • Presents the relevance of mass spectrometry and stable isotope methodology in pediatric research covering applications in Nutrition, Obesity, Metabolic Disorders, and Kidney Disorders • Focuses on the interactions between nutrients and the endogenous metabolism within the body and how these factors affect the health of a growing infant.

Illicit drugs are an emerging class of environmental contaminants and mass spectrometry is the technique of choice for their analysis. This landmark reference discusses the analytical techniques used to detect illicit drugs in wastewater and surface water, details how to estimate the levels of contaminants in the environment, and explores the behavior, fate, and toxic effects of this new class of contaminants, now a ubiquitous presence in wastewater and surface water. The book details how an estimate of illicit drug consumption in a given population can be developed from an analysis of the residues of illicit drugs in wastewater. An important resource for analytical chemists, environmental researchers, forensic scientists, biologists, and toxicologists.