Summarizes research encompassing all of the aspects required to understand, fabricate and integrate enzymatic fuel cells • Contributions span the fields of bio-electrochemistry and biological fuel cell research • Teaches the reader to optimize fuel cell performance to achieve long-term operation and realize commercial applicability • Introduces the reader to the scientific aspects of bioelectrochemistry including electrical wiring of enzymes and charge transfer in enzyme fuel cell electrodes • Covers unique engineering problems of enzyme fuel cells such as design and optimization